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DyLight 549 Streptavidin

Catalog Number: SA-5549

Availability: In stock

$140.00
  • Buy 10 for $126.00 each and save 10%
  • Buy 30 for $112.00 each and save 20%
Unit Size: 1 mg

Astrocytes: Stained for GFAP and detected with Dylight 549 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.* Zoom

Overview

    Features:

    • Can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry
    • Highly purified and possess very low non-specific binding properties
    • Extremely high affinity for biotin
    • Advantages of DyLight® dyes include greater photostability, pH independence, and brighter fluorescence
    • Excitation at 556 nm
    • Emission at 571 nm
    • Color:  Orange

    Additional Information

    Catalog Number SA-5549
    Unit Size 1 mg
    Country of Manufacture United States
    Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
    Conjugate DyLight 549
    Color of Fluorescence Orange
    Format Concentrate
Additional Info

    Details

    Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

    Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

    DyLight® dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight® conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight® conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

    *Additional information about the images can be found here.

     

    DyLight Spectra

    DyLight Table

Protocols/Data Sheet/SDS

    Protocols/Data Sheet/SDS

    SDS Data Sheet

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