Streptavidin, DyLight® 488

SA-5488
SKU Unit Size Price Qty
SA-5488-1 1 mg
$163.00

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Description

DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. 

Features:

  • Advantages of DyLight® dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation maximum: 493 nm
  • Emission maximum: 518 nm
  • Color:  Green

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 493 nm
Maximum Emission 518 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 488
Color of Fluorescence Green
Format Concentrate

Documents

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. 

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight® dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight® conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight® conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

*Additional information about the images can be found here.

 

DyLight Spectra

DyLight Table

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