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DyLight 488 Labeled Lycopersicon Esculentum (Tomato) Lectin (LEL, TL)

Catalog Number: DL-1174

Availability: In stock

$130.00
Unit Size: 1 mg

Mouse kidney, formalin-fixed, paraffin-embedded: DyLight 488 Labeled Lycopersicon Esculentum lectin (green) mounted in VECTASHIELD HardSet with DAPI (blue). Zoom

Overview

    Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

    DyLight® 488 labeled tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

    The excitation maximum is at 493 nm and the emission maximum is at 518 nm (green).

    Additional Information

    Catalog Number DL-1174
    Unit Size 1 mg
    Country of Manufacture United States
    Applications Immunofluorescence, Glycobiology
    Conjugate DyLight 488
    Color of Fluorescence Green
    Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine
Additional Info

    Details

    Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

    Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

    Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.

    DyLight® 488 labeled tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 493 nm and the emission maximum is at 518 nm.

    Inhibiting/Eluting Sugar: Chitin Hydrolysate

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