This product is an electrophoresis standard consisting of 15 bands between 0.2 and 10 kilobase pairs. Bands are regularly spaced so that sizes are easy to identify. Markers are supplied ready-to-use containing loading dye so that electrophoresis can be monitored.
The Biotinylated DNA Molecular Weight Marker (MB-1302) is used when nucleic acids are to be transferred to a membrane and later detected using an avidin or streptavidin-based system.
Ready-to-use marker (5 μl or 500 ng) was loaded on a 0.5% agarose gel. The gel was run in 1x TBE at 10 V/ cm, stained with ethidium bromide (left) or blotted onto nylon membrane using standard methods. Biotinylated DNA bands (right) were detected using the UltraSNAP Kit (Alkaline Phosphatase Streptavidin followed by DuoLuX Chemiluminescent Substrate).
