Streptavidin, CY®5

SA-1500
SKU Unit Size Price Qty
SA-1500-1 1 mg
$174.00

To request a quote for products:

  1. Click “Contact Us” in the header bar above;
  2. Click “Customer Service”;
  3. Complete the form and provide the following information in the “Comments” section: note you would like a quote, item number (SKU) and quantity;
  4. Click “Submit”.

Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. 

  • Excitation:  650 nm
  • Emission:  670 nm
  • Color:  Far Red

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 647 nm
Maximum Emission 663 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate Cy5
Color of Fluorescence Far Red
Format Concentrate

Documents