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Cy3 labeled Concanavalin A (Con A)

Catalog Number: CL-1003

Availability: In stock

$150.00
Unit Size: 1 mg

Tonsil (FFPE):  Antigen retrieved with Antigen Unmasking Solution and stained with Cy3 Con A (red-orange).  Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Zoom

Overview

    Con A recognizes α-linked mannose present as part of a “core oligosaccharide” in many serum and membrane glycoproteins.

    Cy3 labeled Con A has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

    • Excitation: 550 nm
    • Emission: 570 nm
    • Color: Red

    Additional Information

    Catalog Number CL-1003
    Unit Size 1 mg
    Country of Manufacture United States
    Applications Immunofluorescence, Glycobiology
    Conjugate Cy3
    Color of Fluorescence Red
    Sugar Specificity Mannose, Glucose
Additional Info

    Details

    Con A recognizes α-linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins. At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits; below pH 5.6, Con A dissociates into active dimers of 52 kDa. Acetylation, succinylation, or other derivatizations can also produce stable forms with dimeric structures. (See succinylated Con A). Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds.

    Con A requires calcium or manganese ions at each of its four saccharide binding sites. Although these divalent metal ions are bound tightly to the polypeptide structure, buffers which can bind calcium (such as phosphate) generally should be avoided in diluting Con A, since a gradual loss in activity may occur.

    Cy 3 labeled Con A has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

    Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.
    • Excitation: 550 nm
    • Emission: 570 nm
    • Color: Red
    Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside
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