Goat Anti-Rat IgG Antibody (H+L), CY®3

CY-4300

This product has been discontinued.

We have discontinued this product permanently. Please find alternative products from below section.
SKU Unit Size Price
CY-4300-1 1 mg
$162.00

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Description

Cy3 Goat Anti-Rat IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species.   (For mouse tissue applications, please see anti-rat, mouse adsorbed, secondary antibodies.)
  • Recognizes both heavy and light chains (H+L) 
  • Optimally labeled with Cy3 to provide the brightest label for fluorescence microscopy 
  • Supplied in solution 
  • Excitation:  552 nm
  • Emission:  565 nm
  • Color:  Red

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration 1.0 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission 570 nm
Maximum Excitation 550 nm
Recommended Usage The recommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species Rat
Conjugate Cy3
Color of Fluorescence Red
Host Species Goat
Format Concentrate

Documents

Citations

Technical Information

The anti-rat Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays.
 
In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. 
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