Maleimide reagents for labeling thiol groups
These maleimide reagents are designed for labeling proteins, nucleic acids or other molecules containing one or more thiol groups. You can introduce thiol groups into DNA, RNA, or oligonucleotides using the 5' EndTag™ or 3' EndTag™ nucleic acid labeling systems.
In applications involving several steps - from producing and labeling a probe to detecting the labeled probe - assessing labeling efficiency can be an essential part of assay design. The efficiency of labeling can be estimated by comparing the relative detection sensitivity of the labeled nucleic acid to a standardized sample in a side by side dot blot dilution series.