Multi-site Labeling
PHOTOPROBE® Biotin nucleic acid labeling systems
Use these labeling systems when labeling longer strands of nucleic acid (>100bp). These systems:
- Incorporate label at multiple sites along the length of a nucleic acid
- Do not destroy or create a copy of the original nucleic
- Label single-or double-stranded DNA, circular DNA, RNA, siRNA, or PNA (peptide nucleic acid)
These nucleic acid labeling systems use aryl azide chemistry, in which reagents are activated and incorporate into the nucleic acid when exposed to heat or light. The labeling reaction is not base-specific and can be carried out using a mercury vapor bulb, halogen lamp, heating block, or thermal cycler.
- PHOTOPROBE® Biotin — for simple, rapid labeling with biotin.
- PHOTOPROBE® (Long Arm) Biotin — for single-step incorporation of biotin molecules featuring an extra long linker arm, should an extra distance between the sample and tag be needed.
Resources
Nucleic Acid Labeling with PHOTOPROBE® Biotin Kit
PHOTOPROBE® Nucleic Acid Labeling System from Vector Laboratories is the method of choice for simple and rapid incorporation of biotin at multiple sites along the entire length of the nucleic acid. The nucleic acid labeling reaction does not destroy the original nucleic acid nor creates its copy. The integrity of the original sample is preserved. Single or double-stranded DNA, circular DNA, RNA, or PNA (peptide nucleic acid) can be labeled with the same reagents.
FastTag Nucleic Acid Labeling Kit
The FastTag® Nucleic Acid Labeling System from Vector Laboratories is the method of choice for incorporating a label at multiple sites along the entire length of the nucleic acid. The nucleic acid labeling reaction does not destroy the original nucleic acid nor creates its copy. The integrity of the original sample is preserved. Single or double-stranded DNA, circular DNA, RNA, or PNA (peptide nucleic acid) can be labeled with the same reagents.
The FastTag® Nucleic Acid Labeling System utilizes a disulfide-containing universal linker that is incorporated into nucleic acid simply by using heat or light. Reducing the disulfide bond yields a free thiol group. A variety of thiol-reactive haptens, fluorochromes, affinity ligands or other markers can then be covalently bound to the nucleic acid via the FastTag® reagent thiol group. A distinct advantage of the simple setup in each of these labeling options is the ease and economy of scaling up.