Enzyme Polymer Detection Systems

Integrated antibody and enzyme technology for streamlined IHC and western blot detection

Our enzyme polymer detection system integrates secondary antibodies with a high-density micropolymer array of reporter enzyme. This technique:

  • Introduces more enzyme at the site of primary antibody localization 
  • Yields a stronger signal with lower background 
  • Simplifies detection into in a single incubation step 

We make our proprietary enzyme polymer detection reagents without large dextrans or other macromolecules as a backbone. This novel approach allows a higher density of enzymes to access a target with minimal interference.

We offer polymer detection systems for both immunohistochemistry and western blot detection. These products provide:

  • Consistent and sensitive detection 
  • Enhanced signals and diminished background staining (optimum signal:noise ratio)
  • Ready-to-use, pre-diluted reagents in convenient dropper bottles
ImmPRESS® Polymer detection
Our unique polymer conjugation chemistry enables significantly more enzyme to be introduced at the antigen target site, resulting in greater signal intensity and sensitivity.
Enzyme conjugated secondary antibody detection
Standard conjugation methods limit the amount of enzyme available for reactivity and hence result in lower signal intensity and substantially lower sensitivity.

Explore Enzyme Polymer Detection Systems

WestVision™ Peroxidase Polymer antibody conjugates bind primary antibodies of mouse (WB-2000) or rabbit (WB-1000) origin for western blot detection. The high-affinity interaction produces a sharp signal and low background.

ImmPRESS Polymer reagents consist of unique micropolymers of highly active peroxidase or alkaline phosphatase enzyme attached to cross-adsorbed, affinity-purified secondary antibodies. This micropolymer conjugation allows a greater density of enzyme per antibody to bind to the target providing high sensitivity with very low background for IHC tissue staining applications.


Resources

Immunohistochemistry Resource Guide

The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.

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IHC Troubleshooting Guide for Polymer Kits

A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol polymer detection systems.  Also troubleshoot issues of weak or no staining. Laminated.

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ImmPRESS® Polymer Detection Kit for IHC/ICC

The highly sensitive, one-step, ready-to-use, non-biotin, ImmPRESS™ Polymer Detection reagents from Vector Laboratories are the result of novel conjugation methods and micropolymer chemistry. Peroxidase micropolymers allow excellent resolution and crisp, strong staining of antigens, especially nuclear and membrane antigens. ImmPRESS™ reagents avoid the steric problems of other polymer-based reagents, thus providing superior results. These reagents are ideal for multiple antigen labeling because of the simplified one-step protocol.