End Labeling

EndTag™ end-labeling kits

Use Vector’s EndTag™ kits for end labeling of short (<100bp) oligonucleotides. These kits:

• Enable covalent attachment of a variety of fluorescent dyes, haptens, or affinity tags
• Use thiol-specific chemistry to incorporate any labels containing thiol-reactive groups (maleimides, iodoacetamides, etc.)
• Require very little hands-on time in a 1 hour labeling protocol

The 5’ and 3’ EndTag™ kits are ideal in DNA hybridization, PCR, in situ hybridization, binding of capture probes to affinity matrices, and electrophoretic mobility shift assays (EMSA).

• 5’ EndTag™ Labeling Kit — labels 5’ ends of DNA, RNA, or unmodified oligonucleotides.Use it to label PCR primers as labeling of the 5’ end leaves the 3’ end available for polymerization.
• 3’ EndTag™ Labeling Kit — offers simple, uniform labeling of blunt, overlapping, or recessed 3’ ends of DNA. Labeling the 3’ end preserves the 5’ terminal phosphate.