Reagents for biotinylation of carbohydrate groups
You can incorporate biotin into viruses, bacteria, or animal and plant cells via their surface carbohydrate groups. Use Vector’s Biotin (Long Arm) Hydrazide to label carbohydrate groups in a variety of macromolecules.
- Incorporates biotin into sugars containing unsubstituted vicinal hydroxyl groups (usually present at the non-reducing termini of oligosaccharides)
- Spacer arm facilitates access of avidin or streptavidin conjugates to biotin
Couple biotin to carbohydrates in two steps:
- Treat the saccharide briefly with periodate (not included); this produces aldehyde groups on the sugars containing unsubstituted vicinal hydroxyl groups.
- Apply Biotin (Long Arm) Hydrazide, which reacts with the aldehyde groups to produce a stable linkage between the saccharide and biotin.
Applications of Biotin (Long Arm) Hydrazide include:
- Adding biotin to RNA at the 3’ end and to carboxyl groups on proteins and other molecules using a carbodiimide coupling method.
- Activating materials such as plastics with glutaraldehyde for subsequent incorporation of Biotin (Long Arm) Hydrazide onto the surface.