Antigen Retrieval

Antigen unmasking solutions reveal antigens for optimum immunostaining

Use our antigen retrieval reagents with formalin-fixed, paraffin-embedded tissue sections. These reagents — in combination with a high-temperature treatment — unmask antigens for optimum immunolabeling.

Vector Laboratories offers you 2 formulations, each supplied as 100x concentrated stocks.

Lymph Node: Without (left panel) and with (right panel) TRIS-based Antigen Unmasking Solution, Cyclin D1 (rm), ImmPRESS® Anti-Rabbit IgG Kit, DAB (brown) substrate. Hematoxylin QS (blue) counterstain.


Producing immunofluorescence (IF) staining results with high specificity and sensitivity begins with choosing the best reagents. This guide will help you:

  • Learn about the IF workflow and how to optimize each step
  • Quickly identify and choose the most appropriate fluorescent secondary conjugates
  • Select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving