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BLOXALL® Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase

SP-6000-100
SKU Unit Size Price Qty
SP-6000-100 100 ml
$119.00

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Description

BLOXALL Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution is a dual enzyme blocking reagent that inactivates endogenous peroxidase, pseudoperoxidase, and alkaline phosphatase in formalin-fixed, paraffin-embedded tissue sections, frozen tissue sections, and cell preparations. This reagent is provided as a ready-to-use reagent in a convenient dropper bottle.

Features: 

  • More effective than conventional blocking methods
  • Single 10 minute incubation step
  • 100 ml solution

Specifications

More Information
Unit Size 100 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Neurobiology
Recommended Storage 2-8 °C
Recommended Usage Prior to the application of primary antibody, apply BLOXALL™ Blocking Solution to tissue sections or cell preparations and incubate for 10 minutes at room temperature.Wash sections in an appropriate wash buffer suchas Tris buffer, PBS, or TBS for 5 minutes.Incubation time can be shortened based on individual application. Appropriate care should be exercised when using this reagent to avoid contact with eyes and skin.Dispose in accordance with local regulations.
Blocking Action Endogenous Peroxidase, Endogenous Alkaline Phosphatase

Documents

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Citations

Technical Information

Tissues can contain endogenous peroxidase, pseudoperoxidase, and/or alkaline phosphatase activity that will produce background staining if an alkaline phosphatase and/or peroxidase detection system and corresponding substrates are used. 

Home Enzyme Blocking Reagents BLOXALL® Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase