Posts tagged 'Immunofluorescence'

5 reasons to use the ImmEdge PAP Pen in your immunostaining workflow

In this blog post, we cover 5 reasons you should add the ImmEdge® Hydrophobic Barrier PAP Pen from Vector Laboratories to your list of essential lab tools. 

November 16, 2022

My staining didn’t work, part 3: IHC and IF staining tips

This blog will discuss some miscellaneous factors to focus on when an IHC or IF protocol performs unexpectedly.

October 19, 2022

My staining didn’t work, part 2: Absence of positive staining in IHC and IF

One pain for IHC and IF staining can be the lack of positive staining on your tissue sections. In this blog post, we will offer tips and tricks for overcoming the absence of positive staining in IHC and IF. 

October 12, 2022

My staining didn't work, part 1: Background staining in IHC and IF

In this post we will discuss common sources of background staining and offer tips and tricks for resolving these issues.

October 5, 2022

How to protect your tissue from photobleaching

This blog post will walk you through strategies in four different categories to protect your immunofluorescent tissue section from photobleaching.

September 14, 2022

How to choose antifade mounting media

As far as antifade mounting media go, one-size-fits-all solutions are few and far between. Here, we break down key factors you should consider when choosing the best antifade mounting medium for your experiment.

August 10, 2022

5 sources of non-specific staining and background in IHC and IF

Identifying sources of non-specific staining is crucial for determining potential solutions to improve your staining results. This blog post will briefly walk you through commonly encountered sources of non-specific staining in IHC and IF applications.

June 8, 2022

How to prevent tissue and reagent loss on your slides

You spend a lot of time collecting and preparing your tissue samples. Learn tips and tricks to ensure those samples are evenly stained and retained on your slides.

May 4, 2022

Setting media vs non-setting mounting media: Which is right for you?

You’ve got lots of options when it comes to choosing a mounting media. Let us help you decide whether setting or non-setting mounting media will work best for your workflow in this Tips & Tricks article.

April 27, 2022

How to improve your immunofluorescence by overcoming autofluorescence

Interfering background signal, such as autofluorescence, which is fluorescence inherent or endogenous to tissue, cell, or due to certain fixatives, can hamper your ability to accurately distinguish real target antigen expression. In a recent webinar, Dr. Craig Pow discussed common causes of target signal interference, controls to help distinguish background noise from true signal, ways to reduce or eliminate autofluorescence, case studies of autofluorescence quenching, and tips to preserve immunofluorescent signal, such as through the use of Vector® TrueVIEW® Autoflurorescence Quenching Kit. You can watch the full on-demand webinar or keep reading for tips and tricks from the Q&A session.

March 2, 2022