Posts tagged 'Immunofluorescence'

5 sources of non-specific staining and background in IHC and IF

Identifying sources of non-specific staining is crucial for determining potential solutions to improve your staining results. This blog post will briefly walk you through commonly encountered sources of non-specific staining in IHC and IF applications.

June 8, 2022

Setting media vs non-setting mounting media: Which is right for you?

You’ve got lots of options when it comes to choosing a mounting media. Let us help you decide whether setting or non-setting mounting media will work best for your workflow in this Tips & Tricks article.

April 27, 2022

How to improve your immunofluorescence by overcoming autofluorescence

Interfering background signal, such as autofluorescence, which is fluorescence inherent or endogenous to tissue, cell, or due to certain fixatives, can hamper your ability to accurately distinguish real target antigen expression. In a recent webinar, Dr. Craig Pow discussed common causes of target signal interference, controls to help distinguish background noise from true signal, ways to reduce or eliminate autofluorescence, case studies of autofluorescence quenching, and tips to preserve immunofluorescent signal, such as through the use of Vector® TrueVIEW® Autoflurorescence Quenching Kit. You can watch the full on-demand webinar or keep reading for tips and tricks from the Q&A session.

March 2, 2022

Establishing lifelong connections, building a new lab, and repurposing therapeutics

Abdelrahman “Abdel” Fouda officially launched his long-term academic career as an Assistant Professor at the University of Arkansas for Medical Sciences (Little Rock, Arkansas) in the middle of July 2021. Since then, Abdel has been converting an empty lab with just the structural bare-bones basics, recruiting research technicians and postdoctoral fellows, preparing manuscripts based on past research, and writing grants to fund future projects.
February 16, 2022

Easy as Do-Re-Mi: Getting Started with Multiplex Immunohistochemistry

Are you eager to add multiplex immunohistochemistry to your experimental repertoire, but feeling unsure about how to begin? We’re here to help with this tips and tricks article.
January 12, 2022

Our Top 10 Posts of 2021 Will Lend You a Helping Hand in the Lab

How can we do even more to help researchers?  That’s a question we ask a lot here at Vector Laboratories, and this June it led us to launch a blog. Since then, it’s been our honor to bring you stories about innovative scientists and share insights to help you advance your research. Here are a few of our most popular posts from 2021, and we’re looking forward to many more tips and stories in 2022!

December 15, 2021

Following the developmental pathway to improved treatments

Christian Bonatto Paese developed a passion for biology as a youth and is now in the midst of a fellowship in the Division of Plastic Surgery at the Cincinnati Children’s Hospital. His goal is to aid in the development of pharmacological interventions for birth defects in exchange for the more invasive and repetitive surgical solutions that are available today.
November 10, 2021

Celebrating the art of scientific discovery

Join us as we highlight previous winners of Vector Laboratories Microscopy Image Contest. Experience their scientific artwork and learn how you can become part of the scientific art collective.
November 4, 2021

Reduce Tissue Autofluorescence and Dramatically Enhance Signal-to-Noise Ratio

Autofluorescence can make it difficult or impossible to distinguish antigen-specific signal from non-specific background. Learn how you can reduce autofluorescence in your immunofluorescence workflow.
October 27, 2021

Histology in a nutshell: process it, cut it, put it on a slide, and see what it reveals

Colin Nixon, manager of the Histology core lab at the Beatson Institute for Cancer Research in Glasgow Scotland, failed biology at school and was described by most of his teachers as “academically challenged.” They likely would be surprised to see how Colin serendipitously “fell into” the sciences through a government youth training scheme that indicated he had an aptitude for the science, and he has since made a career of it.
October 13, 2021