Identifying sources of non-specific staining is crucial for determining potential solutions to improve your staining results. This blog post will briefly walk you through commonly encountered sources of non-specific staining in IHC and IF applications.
Posts tagged 'Immunofluorescence'
You’ve got lots of options when it comes to choosing a mounting media. Let us help you decide whether setting or non-setting mounting media will work best for your workflow in this Tips & Tricks article.
Interfering background signal, such as autofluorescence, which is fluorescence inherent or endogenous to tissue, cell, or due to certain fixatives, can hamper your ability to accurately distinguish real target antigen expression. In a recent webinar, Dr. Craig Pow discussed common causes of target signal interference, controls to help distinguish background noise from true signal, ways to reduce or eliminate autofluorescence, case studies of autofluorescence quenching, and tips to preserve immunofluorescent signal, such as through the use of Vector® TrueVIEW® Autoflurorescence Quenching Kit. You can watch the full on-demand webinar or keep reading for tips and tricks from the Q&A session.
How can we do even more to help researchers? That’s a question we ask a lot here at Vector Laboratories, and this June it led us to launch a blog. Since then, it’s been our honor to bring you stories about innovative scientists and share insights to help you advance your research. Here are a few of our most popular posts from 2021, and we’re looking forward to many more tips and stories in 2022!