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SpeakEasy Science Blog

Tips Tricks 8

The Waiting Game: A Search for a Streamlined IHC Protocol

Picture this: a chemist walks into a lab and asks his colleagues how to transition some immunohistochemistry (IHC)-stained slides in a buffer solution to coverslip with a non-aqueous mounting media. From his perspective, it’s a simple problem, and it should have a similarly simple solution. Does it? Read this blog article to find out.

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Scientist Spotlight 09

There’s a New Mountant in Town

Earlier this year, Brittany Cederstrom, a research scientist at the Proteogenomic Research Institute for Systems Medicine (PRISM) in San Diego, California sampled VectaMount® Express Mounting Medium in her slide preparation workflow and compared it to her standard process with a competitor mounting medium.

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Scientist Spotlight 11

The Life of a Histotechnologist: One Thin Section

Eric Peterson has long held an interest in the underpinnings of life, found enjoyment in science fiction, and taken a practical approach to career choices rooted in economics. He is not the guy who plotted his career path from start-to-finish; instead, he chose a career in the growing field of technology and was confident that there was a stable market for scientific professionals.

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Tips Tricks 10

Dial up the Contrast with Counterstaining

When scientists detect targets of interest with immunohistochemistry (IHC), antibody binding and substrate detection alone often produce a perplexing picture where the target lacks context. Luckily there’s a solution. Counterstaining IHC samples after enzymatic detection spatially orients the target within its surroundings, which can help guide researchers as they analyze their results.

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Tips Tricks 11 1

A Simple Guide to Immunohistochemistry Substrate Selection

When it comes to selecting the substrate for your IHC application, you have variety and flexibility, and our focus is to help you make the right decision. This blog discusses different factors to consider—enzyme, sensitivity, color, method of visualization, and stability—when selecting the appropriate substrate (and, consequently, color) for your experiment.

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