SpeakEasy Science Blog

How to improve your immunofluorescence by overcoming autofluorescence

Interfering background signal, such as autofluorescence, which is fluorescence inherent or endogenous to tissue, cell, or due to certain fixatives, can hamper your ability to accurately distinguish real target antigen expression. In a recent webinar, Dr. Craig Pow discussed common causes of target signal interference, controls to help distinguish background noise from true signal, ways to reduce or eliminate autofluorescence, case studies of autofluorescence quenching, and tips to preserve immunofluorescent signal, such as through the use of Vector® TrueVIEW® Autoflurorescence Quenching Kit. You can watch the full on-demand webinar or keep reading for tips and tricks from the Q&A session.

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Leveraging Lectins for Glycan Analysis

Incorporating glycan analysis into your workflows is easier than you may think–especially when you use lectins, which are compatible with many commonly used lab assays. Join us as we walk through the key considerations to help you get started.

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