Enhancing the signal-to-noise ratio is essential for obtaining viable data. Choosing the appropriate immunohistochemistry detection system can help.
Find out how two brilliant scientists are studying complex carbohydrate chains to fight cancer in this article about glycobiology and tumorigenesis.
Interfering background signal, such as autofluorescence, which is fluorescence inherent or endogenous to tissue, cell, or due to certain fixatives, can hamper your ability to accurately distinguish real target antigen expression. In a recent webinar, Dr. Craig Pow discussed common causes of target signal interference, controls to help distinguish background noise from true signal, ways to reduce or eliminate autofluorescence, case studies of autofluorescence quenching, and tips to preserve immunofluorescent signal, such as through the use of Vector® TrueVIEW® Autoflurorescence Quenching Kit. You can watch the full on-demand webinar or keep reading for tips and tricks from the Q&A session.
Choosing the appropriate immunohistochemistry detection reagent can be a daunting task. Here are some considerations to guide you on your path to staining success.
When it comes to IHC and IF a strong start is essential to great results. Explore our tips to help your sample preparation process.
Are you eager to add multiplex immunohistochemistry to your experimental repertoire, but feeling unsure about how to begin? We’re here to help with this tips and tricks article.
Adding multiple antibodies to your IHC protocol can lead to stunning scientific insights, but is sometimes technically challenging. Get answers to your multiplex immunostaining quandaries from Dr. Craig Pow in this tips and tricks article.