All about VECTASTAIN® ABC Kits for immunodetection
Avidin-Biotin Complex, or ABC-based detection, is one of the most widely used methods for staining. This method exploits the high affinity exhibited between the protein avidin and the vitamin biotin forming large macromolecular complexes containing multiple enzyme molecules—either horse radish peroxidase (HRP) or alkaline phosphatase (AP).