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Biotinylated Ricinus Communis Agglutinin I (RCA I, RCA120)

Catalog Number: B-1085

Availability: In stock

$145.00
Unit Size: 5 mg

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Overview

     This lectin consists of two subunits of 60 kDa which can be dissociated by reducing agents into closely related chains between 27 kDa and 33 kDa. One of the chains appears to be common to the “B” chain of another castor bean lectin, ricin, while the other chain is unique to RCA I.  The B chain binds to galactose or N-acetylgalactosamine residues of membrane glycoconjugates.

    Biotinylated RCA I has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.

    Additional Information

    Catalog Number B-1085
    Unit Size 5 mg
    Country of Manufacture United States
    Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs
    Conjugate Biotinylated
    Sugar Specificity Galactose, Lactose
Additional Info

    Details

    This lectin consists of two subunits of 60 kDa which can be dissociated by reducing agents into closely related chains between 27 kDa and 33 kDa. One of the chains appears to be common to the B chain of another castor bean lectin, ricin, while the other chain is unique to RCA I.

    Biotinylated RCA I has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.

    This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

    Inhibiting/Eluting Sugar: 200 mM galactose or lactose
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