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Biotinylated Narcissus Pseudonarcissus (Daffodil) Lectin (NPL, NPA)

Catalog Number: B-1375

Availability: In stock

$135.00
  • Buy 10 for $121.50 each and save 10%
  • Buy 30 for $108.00 each and save 20%
Unit Size: 2 mg

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Overview

    NPL, isolated from daffodil bulbs, has a specificity toward α-linked mannose, preferring polymannose structures containing (α-1,6) linkages. Binding to mannose polymers can occur via internal mannose residues and is not dependent on structural integrity of a non-reducing end sugar. NPL also binds some galactomannans, and differs in other binding characteristics from a related lectin, Galanthus nivalis lectin. Unlike Con A, LCA, or PSA, NPL does not bind glucose.

    Biotinylated Narcissus pseudonarcissus lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.

    Additional Information

    Catalog Number B-1375
    Unit Size 2 mg
    Country of Manufacture United States
    Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
    Conjugate Biotinylated
    Sugar Specificity Mannose
Additional Info

    Details

    This lectin exists as a tetramer at neutral pH but below pH 5.0 and above pH 9.0, NPL dissociates into monomers. NPL, isolated from daffodil bulbs, has a specificity toward α-linked mannose, preferring polymannose structures containing (α-1,6) linkages. Binding to mannose polymers can occur via internal mannose residues and is not dependent on structural integrity of a non-reducing end sugar. NPL also binds some galactomannans, and differs in other binding characteristics from a related lectin, Galanthus nivalis lectin. Unlike Con A, LCA, or PSA, NPL does not bind glucose.

    Biotinylated Narcissus pseudonarcissus lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.

    This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

    Inhibiting Sugar: 400 mM α-methylmannoside
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