|Unit Size||0.5 mg|
|Country of Manufacture||United States|
|Applications||Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs|
Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.
Biotinylated Horse Anti-Mouse IgG, rat adsorbed, is supplied in lyophilized form and can be reconstituted with 1 ml water. With some exceptions the recommended dilution for most applications is 1:100.
(H+L) indicates the antibody recognizes both heavy and light chains.
This specially prepared antibody is designed to be used on tissues that may contain interfering endogenous immunoglobulins. The rat adsorbed anti-mouse IgG (H+L) products are designed for use with rat tissues that may contain endogenous rat immunoglobulins. This adsorbed antibody allows primary antibodies made in mouse to be used on rat tissues.