Biotinylated Hippeastrum Hybrid (Amaryllis) Lectin (HHL, AL)

B-1385
SKU Unit Size Price Qty
B-1385-2 2 mg(s)
$149.00

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Description

Amaryllis lectin binds only α-mannose residues, unlike Con A, LCA and PSA that also bind α-glucosyl structures. Like NPL there appears to be an extended binding site for polymannose structures, not requiring mannose to be at the non-reducing terminus. HHL binds both (α-1,3) and (α-1,6) linked mannose structures, as well as some yeast galactomannans. 

Biotinylated Hippeastrum hybrid lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

More Information
Unit Size 2 mg(s)
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer.
Recommended Storage 2-8 °C
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide
Concentration 2 mg active conjugate/ml
Conjugate Biotinylated
Sugar Specificity Mannose

Documents

Product FAQs

I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is meet. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Technical Information

This lectin is composed of four subunits of identical size and is immunologically related to GNL and NPL.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting Sugar: 100 mM α-methylmannoside