Biotinylated Hippeastrum Hybrid (Amaryllis) Lectin (HHL, AL)

Cat. No: B-1385
Price: $131.00
Unit Size: 2 mg(s)

Overview

Amaryllis lectin binds only α-mannose residues, unlike Con A, LCA and PSA that also bind α-glucosyl structures. Like NPL there appears to be an extended binding site for polymannose structures, not requiring mannose to be at the non-reducing terminus. HHL binds both (α-1,3) and (α-1,6) linked mannose structures, as well as some yeast galactomannans. 

Biotinylated Hippeastrum hybrid lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Cat. No. B-1385-2
Unit Size 2 mg(s)
Country of Manufacture United States
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Conjugate Biotinylated
Color of Fluorescence N/A
Sugar Specificity Mannose

More Information

This lectin is composed of four subunits of identical size and is immunologically related to GNL and NPL. Amaryllis lectin binds only α-mannose residues, unlike Con A, LCA and PSA that also bind α-glucosyl structures. Like NPL there appears to be an extended binding site for polymannose structures, not requiring mannose to be at the non-reducing terminus. HHL binds both (α-1,3) and (α-1,6) linked mannose structures, as well as some yeast galactomannans.

Biotinylated Hippeastrum hybrid lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting Sugar: 100 mM α-methylmannoside