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Biotinylated Dolichos Biflorus Agglutinin (DBA)

Catalog Number: B-1035

Availability: In stock

$180.00
  • Buy 10 for $162.00 each and save 10%
  • Buy 30 for $144.00 each and save 20%
Unit Size: 5 mg

Colon:  Dolichos biflorus agglutinin (black, DAB/Ni), Alcian blue counterstain. Zoom

Overview

    This lectin has a carbohydrate specificity toward α-linked N-acetylgalactosamine.  It has been used to establish secretor status in blood group A individuals by hemagglutination inhibition techniques and for blood typing. This lectin has also been used as a general marker of developing renal collecting ducts.

    Biotinylated Dolichos biflorus agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.

    Additional Information

    Catalog Number B-1035
    Unit Size 5 mg
    Country of Manufacture United States
    Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
    Conjugate Biotinylated
    Sugar Specificity N-Acetylgalactosamine
Additional Info

    Details

    Dolichos biflorus agglutinin is a glycoprotein with a molecular weight of about 111 kDa and consists of 4 subunits of approximately equal size. This lectin has a carbohydrate specificity toward α-linked N-acetylgalactosamine. It has been used to establish secretor status in blood group A individuals by hemagglutination inhibition techniques and for blood typing. This lectin has also been used as a general marker of developing renal collecting ducts.

    Biotinylated Dolichos biflorus agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.

    This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

    Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine
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