Used as anterograde tracers, our Biotinylated Dextran Amines (BDA) are of approximately 10,000 MW and are conjugated with either fluorescein or Texas Red®. These tracers can be effectively introduced by iontophoretic or pressure injection methods. The injection site can then be examined easily with the fluorescent microscope while details of labeled fibers and terminals can be observed after VECTASTAIN® ABC - Peroxidase and substrate (DAB, Vector® SG, Vector® VIP, or Vector® NovaRED) development.
| Unit Size | 10 mg |
|---|---|
| Maximum Emission | 514-521 nm |
| Recommended Storage | 2-8 °C, or after reconstitution, store frozen. |
| Recommended Usage | Reconstitute by addition of 0.4 ml of glass distilled water.The reconstituted product will have the following composition: 25 mM NaCl, pH 8.0.No preservative has been added. |
| Molecular Weight | 10000 |
| Maximum Excitation | 495-500 nm |
| Neuronal Tracer - Direction of Transport | Anterograde |
| Detection Method | Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence |
In addition, these BDAs can be used in double labeling protocols in conjunction with anterograde or retrograde HRP labeling or combined with immunohistochemistry for other substances.
C.L. Veenman, et. al. J. Neurosci. Methods, 41 (1992) 239-254
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