Biotinylated Agaricus Bisporus Lectin (ABL)

Cat. No: B-1425
Price: $136.00
Unit Size: 1 mg

Overview

Agaricus bisporus lectin (ABL) isolated from the edible white button mushroom, is composed of two to four very similar isolectins, each a tetramer of approximately 17 kD subunits.  This lectin binds the Thomsen-Friedenreich antigen, also called T disaccharide, galactosyl (β-1,3) N-acetylgalactosamine.  Unlike peanut agglutinin, which does not bind sialylated T antigen, ABL binds either sialylated or asialylated forms.  Also, whereas peanut agglutinin has a proliferative effect on cancer cells, ABL is anti-proliferative, apparently without cytotoxicity.  ABL binds all blood groups and O-linked glycans of IgA subclasses.  Simple mono- and disaccharides are only marginally inhibitory.

Biotinylated Agaricus bisporus lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.

Cat. No. B-1425-1
Unit Size 1 mg
Country of Manufacture United States
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Conjugate Biotinylated
Color of Fluorescence N/A
Sugar Specificity Gal(b-1,3)GalNAc (T disaccharide)

More Information

Agaricus bisporus lectin (ABL) isolated from the edible white button mushroom, is composed of two to four very similar isolectins, each a tetramer of approximately 17 kD subunits.  This lectin binds the Thomsen-Friedenreich antigen, also called T disaccharide, galactosyl (β-1,3) N-acetylgalactosamine.  Unlike peanut agglutinin, which does not bind sialylated T antigen, ABL binds either sialylated or asialylated forms.  Also, whereas peanut agglutinin has a proliferative effect on cancer cells, ABL is anti-proliferative, apparently without cytotoxicity.  ABL binds all blood groups and O-linked glycans of IgA subclasses.  Simple mono- and disaccharides are only marginally inhibitory.


Biotinylated Agaricus bisporus lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Protocols/Data Sheets/SDS