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Biotinylated Agaricus Bisporus Lectin (ABL)

Catalog Number: B-1425

Availability: In stock

$125.00
  • Buy 10 for $112.50 each and save 10%
  • Buy 30 for $100.00 each and save 20%
Unit Size: 1 mg
Overview

    Agaricus bisporus lectin (ABL) isolated from the edible white button mushroom, is composed of two to four very similar isolectins, each a tetramer of approximately 17 kD subunits.  This lectin binds the Thomsen-Friedenreich antigen, also called T disaccharide, galactosyl (β-1,3) N-acetylgalactosamine.  Unlike peanut agglutinin, which does not bind sialylated T antigen, ABL binds either sialylated or asialylated forms.  Also, whereas peanut agglutinin has a proliferative effect on cancer cells, ABL is anti-proliferative, apparently without cytotoxicity.  ABL binds all blood groups and O-linked glycans of IgA subclasses.  Simple mono- and disaccharides are only marginally inhibitory.

    Biotinylated Agaricus bisporus lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.

    Additional Information

    Catalog Number B-1425
    Unit Size 1 mg
    Country of Manufacture United States
    Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
    Conjugate Biotinylated
    Sugar Specificity Gal(b-1,3)GalNAc (T disaccharide)
Additional Info

    Details

    Agaricus bisporus lectin (ABL) isolated from the edible white button mushroom, is composed of two to four very similar isolectins, each a tetramer of approximately 17 kD subunits.  This lectin binds the Thomsen-Friedenreich antigen, also called T disaccharide, galactosyl (β-1,3) N-acetylgalactosamine.  Unlike peanut agglutinin, which does not bind sialylated T antigen, ABL binds either sialylated or asialylated forms.  Also, whereas peanut agglutinin has a proliferative effect on cancer cells, ABL is anti-proliferative, apparently without cytotoxicity.  ABL binds all blood groups and O-linked glycans of IgA subclasses.  Simple mono- and disaccharides are only marginally inhibitory.


    Biotinylated Agaricus bisporus lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.

    This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative.

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Protocols/Data Sheet/SDS

    Protocols/Data Sheet/SDS

    SDS Data Sheet