Western Blotting Workflow

Western blotting is a technique that is often used in the purification, characterization, and identification of proteins. Performing a western blot involves separating proteins by gel electrophoresis and transferring the separated proteins to a blotting membrane. Once blotted, protein can then be detected either directly (using a labeled primary antibody) or indirectly (using a primary … Continue reading Western Blotting Workflow