I have a bottle of the antigen unmasking solution, H-3300. It has been stored in the fridge for a few weeks. When I opened it, it contained some precipitate. Do you know what this precipitate is, and how it may affect product performance?
Product H-3300 is supplied as a highly concentrated (100x) salt solution. It is recommended to be stored in the fridge. In some cases, over time with cold storage, some salts may come out of solution and appear as particulate or precipitated material. Our recommendation is to gently warm the bottle in a warm water bath to re-dissolve the precipitate. Usually 30 min at 35 °C would be sufficient. Once re-dissolved, an aliquot can be drawn from this solution and diluted according to the instructions. Re-dissolving the precipitate maintains the desired pH and salt concentration for optimal performance.