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R.T.U. Animal-Free Blocker and Diluent (AFB)

Catalog Number: SP-5035

Availability: In stock

Unit Size: 100 ml

Prostate without and with R.T.U. Animal Free Block and Diluent used in the blocking step. Both sections are no primary antibody controls with Biotinylated Universal Secondary Antibody, VECTASTAIN® Elite® ABC, and ® Vector DAB Substrate for detection. Note significant toning in section without RTU Animal Free Block and Diluent. 


    R.T.U. Animal Free Blocker™ and Diluent is a ready-to-use solution designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This product contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations where these or other traditional blocking agents are not effective.

    The R.T.U. Animal Free Blocker™ and Diluent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used. 

    Additional Information

    Catalog Number SP-5035
    Unit Size 100 ml
    Country of Manufacture United States
    Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot
    Blocking Action Non-Specific Protein Blocking
    Format Ready-to-Use
Additional Info


    This plant protein derived product is a universal antibody diluent and blocking reagent intended for cell and tissue based immunohistochemistry (IHC) and immunofluorescent (IF) applications. This ready-to-use solution can be used as an alternative to normal sera, BSA, casein and non-fat dry milk in methods where these or other blocking agents have been found to be ineffective.

    It is supplied without any sodium azide, and as such, can be used with both peroxidase and alkaline phosphatase antibody conjugates and all secondary detection reagents including polymer systems and avidin/biotin reagents that incorporate these enzymes. This makes the blocking solution especially convenient in multiplex (multiple antigen labeling) IHC applications where antibodies from different species and a variety of detection systems are used on the same tissue section.

    Importantly, this is a different formulation than our concentrated (5x) animal free blocker, due to the optimized conditions and neutral pH of the solution for IHC and IF methods. 


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Protocols/Data Sheet/SDS

    Protocols/Data Sheet/SDS

    SDS Data Sheet