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AMCA Rabbit Anti-Goat IgG Antibody

Catalog Number: CI-5000

Availability: In stock

$145.00
  • Buy 10 for $130.50 each and save 10%
  • Buy 30 for $116.00 each and save 20%
Unit Size: 1.5 mg

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Overview

    Features:

    • Affinity-purified, ultrapure, high affinity antibody
    • Suitable for use with goat, sheep, and bovine IgG primary antibodies 
    • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species 
    • Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L) 
    • Optimally labeled with AMCA to provide the brightest label for fluorescence microscopy 
    • Supplied in solution 
    • Excitation: 350 nm 
    • Emission: 450 nm 
    • Color: Blue

    Product Citations:

    Additional Information

    Catalog Number CI-5000
    Unit Size 1.5 mg
    Country of Manufacture United States
    Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
    Target Species Goat
    Conjugate AMCA
    Color of Fluorescence Blue
    Host Species Rabbit
    Format Concentrate
Additional Info

    Details

    The rabbit anti-goat IgG antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. Unless otherwise specified, our antibodies will recognize both heavy and light chains (H+L).

    AMCA (7-amino-4-methylcoumarin-3-acetic acid) labeled Rabbit Anti-Goat IgG Antibody excites in the near ultraviolet (350 nm) and emits a visible bright blue fluorescence at about 450 nm. This antibody has been optimally labeled with AMCA to provide the brightest label for fluorescence microscopy.

    As well as recognising goat IgG, this antibody is suitable to detect sheep and bovine IgG primary antibodies.

    The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker solution (Cat. No. SP-5030) is recommended.
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Protocols/Data Sheet/SDS

    Protocols/Data Sheet/SDS

    Data Sheet SDS