- Affinity-purified, ultrapure, high affinity antibody
- Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
- Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L)
- Optimally labeled with AMCA to provide the brightest label for fluorescence microscopy
- Supplied in solution
- Excitation: 350 nm
- Emission: 450 nm
- Color: Blue
|Country of Manufacture
||Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
|Color of Fluorescence
The anti-mouse Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. Unless otherwise specified, our antibodies will recognize both heavy and light chains (H+L).
AMCA (7-amino-4-methylcoumarin-3-acetic acid) labeled Horse Anti-Mouse IgG Antibody excites in the near ultraviolet (350 nm) and emits a visible bright blue fluorescence at about 450 nm. This antibody has been optimally labeled with AMCA to provide the brightest label for fluorescence microscopy.