Goat Anti-Rabbit IgG Antibody (H+L), AMCA

CI-1000
SKU Unit Size Price Qty
CI-1000-1.5 1.5 mg
$169.00

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Description

AMCA Goat Anti-Rabbit IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Optimally labeled with AMCA to provide the brightest label for fluorescence microscopy
  • Supplied in solution
  • Excitation: 350 nm
  • Emission: 450 nm
  • Color: Blue

Specifications

More Information
Unit Size 1.5 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Concentration 1.5 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission 448-454 nm.
Maximum Excitation 345-355 nm
Recommended Usage Maximum Emission @ 510-520 nm
Target Species Rabbit
Conjugate AMCA
Color of Fluorescence Blue
Host Species Goat
Format Concentrate

Documents

Technical Information

The goat anti-rabbit Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis.