Shopping Cart (0)
$0.00

You have no items in your shopping cart.

Agarose Streptavidin

Catalog Number: SA-5010

Availability: In stock

$150.00
Unit Size: 2 ml

Zoom

Overview

    Features:

    • Can be used to separate biotinylated macromolecules from unbiotinylated materials or for solid phase binding assays
    • Prepared by conjugating streptavidin to heat stable, cross-linked 4% agarose gel beads
    • To ensure minimal steric interference and low nonspecific binding, streptavidin is conjugated through a hydrophilic spacer arm
    • Biotin binding activity of the streptavidin optimally preserved through proprietary coupliing procedure
    • Streptavidin cannot be leached from gel with solutes such as Tris buffer
    • Low non-specific binding activity of gel
    • Protein concentration is 1 mg streptavidin per ml of settled agarose beads
    • Supplied as a 1:1 suspension in buffer
    • Binding capacity is approximately 1 mole biotinylated HRP per mole streptavidin

    Additional Information

    Catalog Number SA-5010
    Unit Size 2 ml
    Country of Manufacture United States
    Applications Affinity Chromatography
    Matrix Conjugate Streptavidin
Additional Info

    Details

    Agarose Streptavidin (SA-5010) is prepared by conjugating streptavidin to heat stable, cross-linked 4% agarose gel beads. To ensure minimal steric interference and low nonspecific binding, streptavidin is conjugated through a hydrophilic spacer arm. The procedure we have developed for coupling streptavidin to agarose preserves the biotin binding activity of the streptavidin. Unlike cyanogen bromide coupling, our procedure does not produce conjugates which can be leached from the gel with solutes such as Tris buffer. Our procedure also does not generate charged groups on the gel that can bind proteins nonspecifically. Protein concentration is 1 mg streptavidin per ml settled agarose beads. The product is supplied as a 1:1 suspension in buffer. The binding capacity is approximately 1 mole biotinylated HRP per mole of streptavidin.

    This product can be used to separate biotinylated macromolecules from unbiotinylated materials or for solid phase binding assays.

Reviews

    Write Your Own Review

    You're reviewing: Agarose Streptavidin

Protocols/Data Sheet/SDS

    Protocols/Data Sheet/SDS

    SDS Data Sheet