3' EndTag DNA End Labeling System

Cat. No: MB-9002
Price: $202.00
Unit Size: 1 kit

Overview

Features:

  • Enables simple and uniform labeling of blunt, overhanging or recessed 3' ends of the DNA. 
  • Choice of diverse labels - fluorophores, biotin, haptens, affinity ligands (not included)

 

Kit components:

The 3' EndTag™ Labeling Kit is designed to perform 20 labeling reactions of up to 0.5 nmols of 3' ends (e.g. about 4.2 μg of a 25 base oligonucleotide) per reaction and includes:

  • Terminal Transferase (TdT)
  • SH-GTP
  • 10x TdT buffer
  • Precipitant

 

Note: Requires thiol-reactive labeling reagent:

  • Biotin (Long Arm) Maleimide 
  • DNP Maleimide
  • Fluorescein (Long Arm) Maleimide
  • Fucose Maleimide
  • Texas Red® Maleimide

Note on Shipping: This item is shipped on ice requiring additional shipping charges.

Cat. No. MB-9002
Unit Size 1 kit
Country of Manufacture United States
Target for Labeling DNA
Required Reactive Group 3' -OH group of DNA
Tag/Group Incorporated Thiol

More Information

End labeling is a favored method for applications where an internal label might interfere with hybridization or sequence specific protein binding.

5' or 3' EndTag™ labeled nucleic acids can be used for applications such as DNA hybridization, PCR, in situ hybridization, the binding of capture probes to affinity matrices, or electrophoretic mobility shift assays (EMSA). Short oligonucleotides are labeled more efficiently with these systems than with other methods. In addition, end labeling of oligonucleotides is an economical alternative to having labels inserted during synthesis.

Both the 5' EndTag™ and the 3' EndTag™ Nucleic Acid Labeling Systems enable the covalent attachment of a variety of fluorescent dyes, haptens, or affinity tags to the respective ends of the nucleic acids using thiol-specific chemistry. Labels containing thiol-reactive groups (maleimides, iodoacetamides, etc.) can easily be incorporated.

Labeling time is about 1 hour with very little hands-on time.

3' EndTag™ Labeling Kit

The 3' EndTag™ System enables simple and uniform labeling of blunt, overhanging, or recessed 3' ends of DNA. 3' end labeling is the preferred method if the terminal phosphate at the 5' end must be preserved.

Labeling using the 3' EndTag™ Labeling Kit is achieved in two steps:

1. Terminal deoxynucleotidyl transferase (TdT) incorporates the thiol-containing nucleotide (SH-GTP) onto the 3' end.
2. Thiolated DNA is coupled with a thiol-reactive label.

Thiol-reactive label is not included in the kit and should be selected separately.

3 EndTag System

Kit Contents:

The kit is designed to perform 20 labeling reactions of up to 0.5 nmols of 3' ends (e.g. about 4.2 μg of a 25 base oligonucleotide) per reaction and includes:

  • terminal transferase (TdT)
  • SH-GTP
  • 10x TdT buffer
  • precipitant

Note on Shipping: This item is shipped on ice requiring additional shipping charges.



Protocols/Data Sheets/SDS