Shopping Cart (0)
$0.00

You have no items in your shopping cart.

3' EndTag DNA End Labeling System

Catalog Number: MB-9002

Availability: In stock

$185.00
Unit Size: 1 kit

Dot blot hybridization comparing the sensitivity of biotinylated oligonucleotides.  The probes were labeled with either the 3'EndTag Kit protocol or a terminal transferase reaction with b-CTP.  The dots were detected with the UltraSNAP Detection Kit. Zoom

Overview

    Features:

    • Enables simple and uniform labeling of blunt, overhanging or recessed 3' ends of the DNA. 
    • Choice of diverse labels - fluorophores, biotin, haptens, affinity ligands (not included)

     

    Kit components:

    The 3' EndTag™ Labeling Kit is designed to perform 20 labeling reactions of up to 0.5 nmols of 3' ends (e.g. about 4.2 μg of a 25 base oligonucleotide) per reaction and includes:

    • Terminal Transferase (TdT)
    • SH-GTP
    • 10x TdT buffer
    • Precipitant

     

    Note: Requires thiol-reactive labeling reagent:

    • Biotin (Long Arm) Maleimide 
    • DNP Maleimide
    • Fluorescein (Long Arm) Maleimide
    • Fucose Maleimide
    • Texas Red® Maleimide

    Note on Shipping: This item is shipped on ice requiring additional shipping charges.

    Additional Information

    Catalog Number MB-9002
    Unit Size 1 kit
    Country of Manufacture United States
    Target for Labeling DNA
    Required Reactive Group 3' -OH group of DNA
    Tag/Group Incorporated Thiol
Additional Info

    Details

    End labeling is a favored method for applications where an internal label might interfere with hybridization or sequence specific protein binding.

    5' or 3' EndTag™ labeled nucleic acids can be used for applications such as DNA hybridization, PCR, in situ hybridization, the binding of capture probes to affinity matrices, or electrophoretic mobility shift assays (EMSA). Short oligonucleotides are labeled more efficiently with these systems than with other methods. In addition, end labeling of oligonucleotides is an economical alternative to having labels inserted during synthesis.

    Both the 5' EndTag™ and the 3' EndTag™ Nucleic Acid Labeling Systems enable the covalent attachment of a variety of fluorescent dyes, haptens, or affinity tags to the respective ends of the nucleic acids using thiol-specific chemistry. Labels containing thiol-reactive groups (maleimides, iodoacetamides, etc.) can easily be incorporated.

    Labeling time is about 1 hour with very little hands-on time.

    3' EndTag™ Labeling Kit

    The 3' EndTag™ System enables simple and uniform labeling of blunt, overhanging, or recessed 3' ends of DNA. 3' end labeling is the preferred method if the terminal phosphate at the 5' end must be preserved.

    Labeling using the 3' EndTag™ Labeling Kit is achieved in two steps:

    1. Terminal deoxynucleotidyl transferase (TdT) incorporates the thiol-containing nucleotide (SH-GTP) onto the 3' end.
    2. Thiolated DNA is coupled with a thiol-reactive label.

    Thiol-reactive label is not included in the kit and should be selected separately.

    3 EndTag System

    Kit Contents:

    The kit is designed to perform 20 labeling reactions of up to 0.5 nmols of 3' ends (e.g. about 4.2 μg of a 25 base oligonucleotide) per reaction and includes:

    • terminal transferase (TdT)
    • SH-GTP
    • 10x TdT buffer
    • precipitant

    Note on Shipping: This item is shipped on ice requiring additional shipping charges.



Reviews

    Write Your Own Review

    You're reviewing: 3' EndTag DNA End Labeling System

Protocols/Data Sheet/SDS

    Protocols/Data Sheet/SDS

    SDS Protocol