3' EndTag™ DNA End Labeling System

MB-9002
SKU Unit Size Price Qty
MB-9002 1 kit
$202.00

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Description

The 3' EndTag™ System enables simple and uniform labeling of blunt, overhanging, or recessed 3' ends of DNA. 3' end labeling is the preferred method if the terminal phosphate at the 5' end must be preserved.

The 3' EndTag™ Nucleic Acid Labeling System enables the covalent attachment of a variety of fluorescent dyes, haptens, or affinity tags to the respective ends of the nucleic acids using thiol-specific chemistry. Labels containing thiol-reactive groups (maleimides, iodoacetamides, etc.) can easily be incorporated.

 

Kit components:

The 3' EndTag™ Labeling Kit is designed to perform 20 labeling reactions of up to 0.5 nmols of 3' ends (e.g. about 4.2 μg of a 25 base oligonucleotide) per reaction and includes:

  • Terminal Transferase (TdT)
  • SH-GTP
  • 10x TdT buffer
  • Precipitant

 

Note: Requires thiol-reactive labeling reagent:

  • Biotin (Long Arm) Maleimide 
  • DNP Maleimide
  • Fluorescein (Long Arm) Maleimide
  • Fucose Maleimide
  • Texas Red® Maleimide

Note on Shipping: This item is shipped on ice requiring additional shipping charges.

Specifications

More Information
Unit Size 1 kit
Target for Labeling DNA
Required Reactive Group 3' -OH group of DNA
Tag/Group Incorporated Thiol
Safety Title WARNING: NA - www.P65Warnings.ca.gov

Kit Components

Kit components:

The 3' EndTag™ Labeling Kit is designed to perform 20 labeling reactions of up to 0.5 nmols of 3' ends (e.g. about 4.2 μg of a 25 base oligonucleotide) per reaction and includes:

  • Terminal Transferase (TdT)
  • SH-GTP
  • 10x TdT buffer
  • Precipitant

Note: Requires thiol-reactive labeling reagent:

  • Biotin (Long Arm) Maleimide
  • DNP Maleimide
  • Fluorescein (Long Arm) Maleimide
  • Fucose Maleimide
  • Texas Red® Maleimide

Documents

Technical Information

End labeling is a favored method for applications where an internal label might interfere with hybridization or sequence specific protein binding.

5' or 3' EndTag™ labeled nucleic acids can be used for applications such as DNA hybridization, PCR, in situ hybridization, the binding of capture probes to affinity matrices, or electrophoretic mobility shift assays (EMSA). Short oligonucleotides are labeled more efficiently with these systems than with other methods. In addition, end labeling of oligonucleotides is an economical alternative to having labels inserted during synthesis.

Labeling time is about 1 hour with very little hands-on time.

3' EndTag™ Labeling Kit

Labeling using the 3' EndTag™ Labeling Kit is achieved in two steps:

1. Terminal deoxynucleotidyl transferase (TdT) incorporates the thiol-containing nucleotide (SH-GTP) onto the 3' end.
2. Thiolated DNA is coupled with a thiol-reactive label.

Thiol-reactive label is not included in the kit and should be selected separately.

3 EndTag System

 

Note on Shipping: This item is shipped on ice requiring additional shipping charges.

 


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