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Featuring:
Plus
quality reagents for:
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| FastTag Basic Nucleic Acid Labeling Kit
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Fluorescence in situ hybridization of human chromosomes using FastTag Fluorescein-labeled pHuR 98 detected indirectly with Biotinylated Anti-Fluorescein and Fluorescein Avidin DCS, and FastTag Texas REd labeled pUC1.77 detected directly.
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The FastTag® Nucleic Acid Labeling Systems are the methods of choice for incorporating a label at multiple sites along the entire length of the nucleic acid. The nucleic acid labeling reaction does not destroy the original nucleic acid nor creates its copy. The integrity of the original sample is preserved. Single or double-stranded DNA, circular DNA, RNA, or PNA (peptide nucleic acid) can be labeled with the same reagents.
DNA/RNA labeling is based on aryl azide chemistry in which the reagent, when exposed to heat or light, becomes activated and incorporates into the nucleic acid without base specificity. The nucleic acid labeling reaction can be carried out using a mercury vapor bulb (sun lamp), a UV lamp which produces light in the 350 nm to 370 nm range, a halogen lamp, a heating block, or a thermal cycler providing the investigator with great flexibility in experimental design.
A distinct advantage of the simple setup in each of these labeling options is the ease and economy of scaling up.
The FastTag® Nucleic Acid Labeling System utilizes a disulfide-containing universal linker that is incorporated into nucleic acid simply by using heat or light. Reducing the disulfide bond yields a free thiol group. A variety of thiol-reactive haptens, fluorochromes, affinity ligands or other markers can then be covalently bound to the nucleic acid via the FastTag® reagent thiol group.
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Kit Contents:
The FastTag® Labeling Kit† contains the following reagents to label up to 250 µg of nucleic acid (or to carry out up to 50 labeling reactions):
FastTag® Reagent
Tris Buffer
Citrate Buffer
Reducing Reagent
sec-Butanol
Precipitant
†Use this kit with any thiol-reactive label.
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Thiol-reactive Labeling Reagents
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FastTag Labeling System Video Tutorial
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Labeling DNA using the FastTag system. Both light and heat activation of the FastTag reagent are demonstrated in this step by step guide. |
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Dot Blot Assay Video Tutorial
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Step by step demonstration of the dot blot technique. |
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Biotin Quantitation using the QuantTag Biotin Kit
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Sensitive method of measuring biotin, free or bound to macromolecules using the QuantTag Kit. |
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