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Featuring:
Plus
quality reagents for:
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Antibodies to Nucleic Acid Labels |
These reagents can be used to detect PHOTOPROBE® Biotin-, FastTag®-, or 5’ EndTag™-labeled probes in applications such as in situ hybridization or Southern and northern blotting. All antibodies have been affinity-purified. Some commonly used labels that are available from Vector Laboratories are:
Biotin – The extraordinary affinity of avidin and streptavidin for
biotin and the many biotin-avidin/streptavidin systems available
make this label ideal for a variety of applications including in
situ hybridization, blotting, and affinity binding.
Digoxigenin - ISH probes are frequently labeled with digoxigenin (DIG), a steroid found in plants. This is particularly common in double label
applications with a biotin-labeled probe. Digoxigenin labeled
probes can be detected using our high affinity, purified
antibody raised against digoxigenin and digoxin.
DNP (Dinitrophenyl) – DNP is not found endogenously in
tissue so it is an excellent alternative to biotin. High affinity,
purified antibodies are available for detection or amplification
of the signal.
Fluorescein – Fluorescein is not found endogenously in
biological systems. This fluorescent label can be visualized
directly (ex 495 nm; em 515 nm) or used as a hapten and
detected with our biotinylated or enzyme-conjugated antibody
to fluorescein.
Texas Red® - Texas Red® is also not found endogenously
in tissues. This fluorescent label is a high quantum yield
rhodamine that can be directly visualized (ex 595 nm; em
615 nm). The label can also be detected, and the signal
amplified, using our biotinylated or alkaline phosphatase
conjugated antibody to rhodamine.
Fucose – This unique label is ideal for reversible binding of
labeled nucleic acid to VECTREX® AAL, a matrix containing the
fucose-specific lectin Aleuria aurantia. Fucose-labeled nucleic
acids can be bound and eluted under mild conditions. Alkaline
phosphatase conjugated Aleuria aurantia lectin can be used
in dot blot applications to assess labeling efficiency with the
fucose label.
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In situ hybridization using a FastTag® DNP-labeled oligonucleotide cocktail with proinsulin mRNA of islet cells on paraffin-embedded, formalin-fixed human pancreatic tissue. Detected using Biotinylated Anti-DNP and Alkaline Phosphatase Streptavidin followed by BCIP/NBT substrate.
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FastTag® Texas Red®-labeled pUC1.77 detected with Biotinylated Anti-Rhodamine and Texas Red® Avidin DCS, and FastTag® DNP labeled pHuR 98 detected with Biotinylated Anti-DNP and Fluorescein Avidin DCS. Counterstained with VECTASHIELD® with DAPI.
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