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DyLight 488 Goat Anti-Rabbit IgG Antibody

Catalog Number: DI-1488

Availability: In stock

$130.00
Unit Size: 1.5 mg

Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.* Zoom

Overview

    Features:

    • Affiinity-purified, ultrapure, high affinity antibody 
    • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species 
    • Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L) 
    • Optimally labeled with DyLight® 488 to provide the brightest label for fluorescence microscopy
    • Supplied in solution 
    • Excitation: 493 nm 
    • Emission: 518 nm 
    • Color: Green

    Additional Information

    Catalog Number DI-1488-1.5
    Unit Size 1.5 mg
    Country of Manufacture United States
    Applications Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
    Target Species Rabbit
    Conjugate DyLight 488
    Color of Fluorescence Green
    Host Species Goat
    Format Concentrate
Additional Info

    Details

    The goat anti-rabbit Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. Unless otherwise specified, our antibodies will recognize both heavy and light chains (H+L).

    DyLight® fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

    DyLight® dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight® dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight® dyes can be applied as single labels or in combination with other DyLight® dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The Dylight® dyes currently offered are DyLight® 488 (green), DyLight® 549 (orange), DyLight® 594 (red), and DyLight® 649 (far red).

     

    DyLight Spectra

    DyLight Table

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Protocols/Data Sheet/SDS

    Protocols/Data Sheet/SDS

    Data sheet SDS
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