Tissues can contain endogenous alkaline phosphatase activity
that will produce background staining if an alkaline phosphatase
detection system and substrate are used. To eliminate this
problem, Levamisole Solution, an inhibitor of alkaline
phosphatase, is provided in a convenient dropper bottle and
can be added to the alkaline phosphatase substrate solution.
Levamisole does not inhibit the isoenzyme used for either the
VECTASTAIN® ABC-AP reagents or other alkaline phosphatase
conjugates. 100x Concentrate.
One drop of levamisole concentrate is added to 5 ml of substrate solution.
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