VECTASTAIN Elite ABC Kit (Standard*)


Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin Labeling Kit and detected with VECTASTAIN Elite ABC Reagent and Vector DAB substrate.

Catalog # Unit Size Product Information MSDS
PK-6100    1 kit   Multiple Antigen Labeling Guide
Protocol
Enzyme Immunoassays, Hybridoma Screening, and Western Blots
Troubleshooting Guide
Enzyme Substrate Combinations Chart
Quenching Endogenous Peroxidase Activity  
PK-6100series.eumsds.pdf  


VECTASTAIN Elite ABC Video Tutorial

  Tissue staining using the VECTASTAIN Elite ABC system. For additional instructional videos please see the Tutorials page under the Support link.
* This kit contains 2 ml concentrated stocks of Reagent A and Reagent B to form the ABC reagent.

With more than 40,000 citations to its credit, the VECTASTAIN® ABC kit remains widely popular. Based on the versatile biotin/avidin interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry laboratory.

Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjuction with the high sensitivity and low background of the VECTASTAIN® ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

VECTASTAIN® Elite® ABC System

  • Advanced avidin/biotin technology: The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target.

  • Highest sensitivity, low background: The VECTASTAIN® Elite® ABC system is the most sensitive avidin/biotin-based peroxidase system. The Elite® ABC series is approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background.

  • Cost effective: Higher sensitivity leads to lower cost per slide.

  • Available without (“Standard” kit) or with biotinylated species specific or universal secondary antibodies.

  • Available in Ready-To-Use (R.T.U.) formats: Prediluted, stabilized working solutions of Elite® ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN® Elite® ABC Kit reagents.
  • Product review on Biocompare.com.

    For additional information on the VECTASTAIN® ABC systems, please click on the link below.
    VECTASTAIN ABC Systems

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