Protein labeling and modification is a powerful tool for
the isolation and analysis of proteins. The ProtOn™ Protein
Labeling Kits simplify the attachment of either biotin or
fluorescein to antibodies or other proteins. Vector Laboratories
offers a variety of reagents and kits for protein labeling
allowing for greater flexibility of experimental design.
This ProtOn™ protein labeling kit is designed for the simple and fast labeling of proteins with fluorescein. The kit is ideally suited to label antibodies, hormones, or other proteins as well as other macromolecules containing primary amino groups. Labeled proteins can be used in a number of applications, such as immunological assays, fluorescence microscopy, investigation of protein-protein interactions, etc.
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ProtOn Fluorescein Protein Labeling Kit
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Labeling proteins with fluorescein. |
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Kit Contents:
Each kit contains all the components needed for at least five labeling reactions (up to 1 mg of protein per reaction). Kit components include:
Labeling Reagent (fluorescein isothiocyanate)
Reagent Solvent
Reaction Buffer
Stop Reagent
Gel Filtration Slurry
5 x 1 ml Spin Columns
5 x 2 ml Collection Tubes
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The ProtOn™ labeling reagents react with primary amines (i.e. lysine residues or terminal amino groups) on the protein, forming a covalent bond. The linkage between the label and the protein produced with these reagents is stable, with no significant loss of the label during storage.
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Each ProtOn™ Kit is supplied with complete instructions that offer the researcher a step-by-step guide to convenient and easy protein labeling with biotin or fluorescein.
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