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Featuring:
Plus
quality reagents for:
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| UltraSNAP Detection Kit for Nucleic Acid Blots
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Chemiluminescent [A] and fluorescent [B] detection of a single copy gene in a yeast genomic digest (lane 1) and a control plasmid DNA (lane 2) using the UltraSNAP™ Kit. Both images were acquired from the same blot.
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The UltraSNAP™ Detection Kit is designed to detect biotinylated
nucleic acids on nylon or nitrocellulose. Biotin can be incorporated
into a nucleic acid probe using PHOTOPROBE® Biotin,
FastTag®, 3’ or 5’ EndTag™ Labeling Systems or other established
enzymatic methods.
The UltraSNAP™ kit consists of Alkaline Phosphatase Streptavidin,
the DuoLuX™ Chemiluminescent/Chemifluorescent
Substrate as well as specially optimized PolyBlock™ Blocking
Reagent and washing solutions formulated to produce high
sensitivity with low background.
Streptavidin coupled to alkaline phosphatase binds to the
biotinylated probe on the blot. The probe is visualized
by the conversion of the DuoLuX™ Chemiluminescent/
Chemifluorescent Substrate to a luminescent and fluorescent
product by alkaline phosphatase. The sensitivity is enhanced
with the use of the PolyBlock™ Blocking Reagent and wash
buffers included in the kit which are specifically designed to
minimize background.
UltraSNAP™ Kit components include:
DuoLuX™ Substrate (100 ml)
Alkaline Phosphatase Streptavidin (600 µl)
10x PolyBlock™ Blocking Reagent (120 ml)
20x Wash A (120 ml)
10x Wash B (120 ml)
One kit contains sufficient reagents to develop approximately twenty 100 cm2 blots.
Chemiluminescence can be documented by exposure to film or by image capture with digital imaging systems. Blots can be exposed to film several times over an extended period of time, with typical film exposure times of 30 seconds to 10 minutes. Sensitivities as low as 100 fg of target nucleic acid can be achieved. Optimal exposure times using digital imaging systems depend on the sensitivity of the instruments. Chemiluminescence can be developed on either nylon or nitrocellulose membranes, although nylon is preferred because of faster signal production.
The fluorescence of the developed substrate can be recorded with a digital imaging system or conventional camera months after the chemiluminescence has faded. Acquiring an image from the fluorescent signal requires a much shorter exposure time than from the chemiluminescent signal, often just a fraction of a second.
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