Prevents rapid loss of fluorescence during microscopic examination
Retains its anti-fading ability during long-term storage
Inhibits photobleaching of Fluorescein, Texas Red®, Rhodamine, AMCA, and other fluorochromes
Unique, stable formula superior to buffered glycerol, polyvinyl alcohol-based mounting solutions, or those containing commonly used anti-fading agents
Optically clear
|
|
VECTASHIELD® Mounting Media are unsurpassed in preventing the rapid loss of fluorescence during microscopic examination. The different formulations of VECTASHIELD® Mounting Media all offer the same outstanding anti-fade and anti-photobleaching properties. They are all compatible with fluorescein, Texas Red®, AMCA and a wide variety of other fluorochromes. Different formulations are available to best suit the investigators' priorities and experimental design.
|
|
The original VECTASHIELD® Mounting Medium is a glycerol-based, aqueous mountant that does not solidify but remains a viscous liquid on the slide. After mounting, coverslipped slides will not readily dry out and can be reviewed for weeks afterwards without sealing. For prolonged storage, coverslips can be permanently sealed around the perimeter with nail polish.
|
|
VECTASHIELD® HardSet™ Mounting Medium is an aqueous mountant that hardens when allowed to set at room temperature for at least 20 minutes. This facilitates handling of the slide, eliminates the need to secure the coverslip, and is more convenient for use with oil immersion microscopy. Although stained and mounted slides may be stored for several weeks, VECTASHIELD® HardSet™ Mounting Medium is not a permanent mounting medium. To remove coverslip and mounting medium, slides can simply be incubated in buffer until the mounting medium is removed. Slides can be remounted in fresh mounting medium.
|
|
Both the VECTASHIELD® HardSet™ and the original VECTASHIELD® Mounting Media are available with or without the counterstain DAPI (4,6'diamidino-2-phenylindole; blue). The original VECTASHIELD® Mounting Medium is also available with the counterstain propidium iodide (PI; red). These counterstains are provided at an optimal concentration of 1.5ug/ml. Lower concentrations can be achieved by dilutions with the appropriate VECTASHIELD® Mounting Medium.
|
|
DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm. PI has a broad excitation range with a maximum at about 535 nm and emission at about 615 nm when bound to DNA.
|
|
Please note: VECTASHIELD® Mounting Medium may not be compatible with all enzymatic substrates or fluorescent proteins and test sections would be advisable when using anything other than commonly used fluorochromes.
|
|
1R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182.
|
|
|
Colon stained with fluorescein labeled Amaranthus caudatus lectin using VECTASHIELD® Mounting Medium
|
|
|
Multiple labeling of colon with Fluorescein (green), Texas Red® (pink/red), and AMCA (blue) using VECTASHIELD® Mounting Medium.
|