What's New! VECTASHIELD® HardSet™ Mounting Medium VECTASHIELD® HardSet™ Mounting Medium provides the same outstanding fluorescence preservation and anti-fade properties as the original VECTASHIELD® Mounting Medium, but it also hardens. No coverslip sealing required! Also available with DAPI. ProtOn™ Protein Labeling Kits The ProtOn™ Protein Labeling Kits are designed for simple and fast labeling of proteins with biotin or fluorsescein. Streptavidin/Biotin Blocking Kit This kit is designed for use with streptavidin conjugates, to block non-specific binding to tissue elements. Streptavidin and biotin are supplied in convenient dropper bottles. Sugars for Lectin Inhibition and Elution Eight different sugars are supplied in dry, preweighed form ready for reconstitution to produce 5 ml or 100 mM or 400 mM solutions. Designed for use as inhibitors of lectin binding to confirm specificity in tissue or cell staining or to elute glycoconjugates from agarose-bound lectins. 5x In Situ Hybridization Blocking Solution A blocking reagent for fluorescent and enzymatic detection systems for In Situ Hybridization and Fluorescent In Situ Hybrdization. 3' EndTag™ Nucleic Acid Labeling System The 3' EndTag™ Labeling System enables simple and uniform labeling of DNA 3' ends. A choice of diverse labels (fluorochromes, haptens, affinity ligands) gives this system outstanding versatility. 3' end labeling is preferred over 5' end labeling if the terminal 5' end needs to be preserved. 3' EndTag-labeled nucleic acids can be used for applications such as DNA hybridization, PCR, in situ hybridization or electrophoretic mobility shift assays. 5' EndTag™ Nucleic Acid Labeling System The 5' EndTag™ Labeling System facilitates the covalent attachment of a variety of fluorescent dyes, haptens, or affinity tags to the 5' end of unmodified oligonucleotides or 5'-OH modified DNA or RNA. 5' EndTag is ideal for labeling PCR and sequencing primers because a label is attached only at the 5' end, leaving the 3' end available for polymerization. The position of the label does not interfere with hybridization or nucleic acid binding and is, therefore, appropriate for binding of capture probes to affinity matrices and for gel shift assays. Labeling requires about 1 hour with very few minutes of hands-on time. Vector® Fusion-Aid™ Kits Vector® Fusion-Aid™ Kits are designed to isolate fusion proteins containing any one of the following "tags": green fluorescent protein, maltose binding protein, c-Myc, or HA. Each kit contains an affinity-purified antibody to the specific "tag" coupled through a stable hydrophilic spacer arm to crosslinked 4% agarose beads. Spin columns and collection tubes are included for convenience. Biotinylated affinity-purified antibodies to fusion protein tags Biotinylated, affinity-purified antibodies to fusion protien tags (green fluorescent protein, maltose binding protein, c-Myc, or HA) are useful for localizing fusion proteins on western blots. The biotin label allows subsequent detection using avidin or streptavidin-based methods (See our VECTASTAIN ABC-AmP kits for western blot detection). A free poster is available featuring examples of single and multiple antigen labeling on tissue sections using immunohistochemistry. Informative enzyme substrate and counterstain compatibility tables complete this concise and educational resource. Please ask us for a complimentary copy. New primary antibodies are available from Novocastra. See what's now available.