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Featuring:
Plus
quality reagents for:
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| Biotin (Long Arm) Hydrazide
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Biotin (Long Arm) Hydrazide (SP-1100) reacts with aldehyde
groups and thus can be used to label carbohydrate groups
present in a wide variety of macromolecules. The spacer
arm in this product allows optimal accessibility of avidin or
streptavidin conjugates to biotin.
Polysaccharides, glycoproteins, or glycolipids. Biotin can
be incorporated into viruses, bacteria, or animal and plant cells
via their surface carbohydrate groups.
Incorporation of Biotin (Long Arm) Hydrazide occurs onto
sugars containing unsubstituted vicinal hydroxyl groups
that are usually present at the non-reducing termini of the
oligosaccharide.
Coupling of biotin is accomplished in two steps:
(1) A brief treatment of the saccharide with periodate (not
included) produces aldehyde groups on the sugars containing
unsubstituted vicinal hydroxyl groups.
(2) Biotin (Long Arm) Hydrazide reacts with the aldehyde groups,
producing a stable linkage between the saccharide and biotin.
RNA 3’ End Labeling. RNA can be specifically labeled with
biotin at the 3’ end. The two-step procedure involves:
(1) Mild oxidation of the ribose located at the 3’ end of RNA,
the only ribose susceptible to periodate oxidation. The bond
between the 2’ and 3’ hydroxyl groups on the ribose is cleaved,
generating two aldehyde groups.
(2) Reaction of both aldehyde groups with Biotin (Long Arm)
Hydrazide, introducing two biotins at the 3’ end.
Proteins and other molecules. This reagent can also be used
to couple biotin to carboxyl groups on proteins and other
molecules using a carbodiimide coupling method.
Other materials. Initial activation of a material such as
plastics with glutaraldehyde allows subsequent incorporation
of Biotin (Long Arm) Hydrazide onto the surface.
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