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Featuring:
Plus
quality reagents for:
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| Agarose Streptavidin
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Agarose Streptavidin (SA-5010) is prepared by conjugating
streptavidin to heat stable, cross-linked 4% agarose gel beads.
To ensure minimal steric interference and low nonspecific
binding, streptavidin is conjugated through a hydrophilic
spacer arm. The procedure we have developed for coupling
streptavidin to agarose preserves the biotin binding activity
of the streptavidin. Unlike cyanogen bromide coupling, our
procedure does not produce conjugates which can be leached
from the gel with solutes such as Tris buffer. Our procedure
also does not generate charged groups on the gel that can
bind proteins nonspecifically. Protein concentration is 1 mg
streptavidin per ml settled agarose beads. The product is
supplied as a 1:1 suspension in buffer. The binding capacity is
approximately 1 mole biotinylated HRP per mole of streptavidin.
This product can be used to separate biotinylated
macromolecules from unbiotinylated materials or for solid phase
binding assays.
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