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Biotinylated Anti-polyHistidine

 

Catalog # Unit Size Product Information MSDS
BA-0705    0.25 mg   Data Sheet   msdsBA0705.pdf  


Biotinylated Anti-polyHistidine antibody is raised against a synthetic peptide containing a 6xHistidine sequence, and binds to N- and C- terminal polyHistidine tags equally well. The biotinylated antibody is to be used in western blot detection of fusion proteins tagged with 6xHsitidine at either terminus. This product has been optimized for use with the VECTASTAIN ABC-AmP Kit (Cat. No. AK-6000). The recommended dilution for use is 1:1000 in 1x casein solution (10x Casein Solution, Cat. No. SP-5020).  
 
Western blot detection of b-galactosidase (108 kD) with a C-terminal polyHistidine tag. The membrane was blocked in 1x casein, incubated in 1:1000 (1 microg/ml) dilution of BA-0705, followed by VECTASTAIN ABC-AmP in 1x casein. The membrane was stained with BCIP/NBT substrate. Lane 1. MW marker. Lane 2-5. Serial dilutions of b-galactosidase.  
 
Western blot detection of Ecotin (18 kD) with an N-terminal polyHistidine tag. The membrane was blocked in 1x casein, incubated in 1:1000 (1 micro/ml) dilution of BA-0705, followed by VECTASTAIN ABC-AmP in 1x casein. The membrane was stained with BCIP/NBT substrate. Lane 1. MW marker. Lanes 2-5. Serial dilutions Ecotin.