Vector Laboratories’ fluorochrome-conjugated streptavidin reagents are highly purified and possess very low nonspecific
binding properties. They have extremely high affinity
for biotin. These fluorescent conjugates can be used to detect
biotinylated secondary antibodies and other macromolecules in
applications such as immunofluorescence, in situ hybridization,
or flow cytometry.
Amplification of fluorescent signals can be easily achieved with
our biotinylated secondary antibodies followed by our highly
purified fluorochrome-labeled streptavidin or avidin. Using a
biotin/avidin or biotin/streptavidin detection system results in
an additional layer of amplification over a directly conjugated
secondary antibody.
Our streptavidin is conjugated using traditional
fluors such as AMCA, fluorescein, phycoerythrin, Texas Red®,
as well as the new DyLight® dyes.
DyLight® dyes offer several advantages including greater
photostability, pH independence, and brighter fluorescence.
We offer DyLight® conjugated streptavidin for use in a
variety of applications, in particular, cell- and tissue-based
immunofluorescent detection. The DyLight® conjugates are
completely stable from pH 4 to pH 9, making them compatible
with many aqueous-based buffers and diluents.
Phycoerythrin is a protein that produces a bright red-orange
fluorescence. Our newly improved Phycoerythrin Streptavidin
(SA-5207) is substantially brighter than previous conjugates.
Phycoerythrin Streptavidin can be used on gene chips, in cell
sorting, and in tissue staining.
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