This lectin is a dimer of two identical subunits of about 36 kDa
each. Unlike Ulex europaeus and Lotus tetragonolobus
lectins which prefer (α -1,2) linked fucose residues, Aleuria
aurantia lectin binds preferentially to fucose linked (α -1,6)
to N-acetylglucosamine or to fucose linked (α -1,3) to
N-acetyllactosamine related structures. AAL also reversibly
binds fucose attached to nucleic acids.
This unique label is ideal for reversibly binding labeled nucleic acid to a matrix. Fucose labeled nucleic acids can be bound and eluted under mild conditions. Alkaline phosphatase Aleuria aurantia can be used in dot blot applications to assess labeling efficiency with the fucose label.
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