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Featuring:
Plus
quality reagents for:
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Chemiluminescence |
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The DuoLuX™ Chemiluminescent and Fluorescent Substrate is a unique formula based on acridan chemistry with very high sensitivity, prolonged light emission, and permanent fluorescence. The DuoLuX™ Chemiluminescent and Fluorescent Substrate is supplied in two forms, for either horseradish peroxidase or alkaline phosphatase development and can be used for western, Southern, northern, or dot blots, or for ELISA applications. This substrate provides sensitivity levels equivalent to about 10 times that achieved with the alkaline phosphatase substrate BCIP/NBT and about 100 times the sensitivity achieved using DAB as a peroxidase substrate.
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The unique long and intense light emission characteristics of the DuoLuX™ Substrate and the option of using either chemiluminescence or fluorescence detection provides both sensitivity and flexibility in development.
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The DuoLuX™ Chemiluminescent/Fluorescent Substrate for peroxidase (Cat. No. SK-6604) is supplied in two bottles, consisting of 100 ml of the DuoLuX™ Substrate (Reagent 1) and 100 ml of a peroxidase converter solution (Reagent 2). Reagents 1 and 2 are mixed in equal volumes just prior to use, providing 200 ml of peroxidase substrate working solution. The DuoLuX™ Chemiluminescent/Fluorescent Substrate for alkaline phosphatase (Cat. No. SK-6605) is supplied in ready-to-use form, consisting of 100 ml of ready-to-use substrate solution.
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Additional Information:
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Application: Western Blot Detection
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Application: Nucleic Acid Detection
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Properties: Chemiluminescent
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Properties: Fluorescent
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