These thiol-reactive labeling reagents are designed for labeling of proteins, nucleic acids or other molecules containing one or more thiol groups. Molecules to be labeled with a maleimide labeling reagent require free thiol groups. In the case of proteins, the maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as primary amines with compounds like Traut's reagent (2-iminothiolane).
These thiol-specific labels can be linked to DNA, RNA, or oligonucleotides using the 3’ EndTag™ Kit, the 5’ EndTag™ Kit, or FastTag® Basic Labeling Kit.
Biotin - Because of the extraordinary affinity of avidin and streptavidin to biotin and the many biotin-avidin/streptavidin systems available, this label is ideal for a variety of applications including in situ hybridization, blotting, and affinity binding.
DNP (Dinitrophenyl) - Because DNP is not found endogenously in tissue, it is an excellent alternative to biotin. High affinity, purified antibodies are available for detection or amplification of the signal.
Fluorescein - This fluorescent label can be directly detected or used as a hapten and detected with our high affinity antibody reagents. (Excitation maximum at 495 nm; emission maximum at 515 nm).
Fucose - This unique label is ideal for reversible binding of labeled nucleic acid to the matrix, VECTREX® AAL. Fucose labeled nucleic acids can be bound and eluted under mild conditions. Alkaline phosphatase Aleuria aurantia lectin can be used in dot blot applications to assess labeling efficiency with the fucose label.
Texas Red® - This fluorescent label is a high quantum yield rhodamine derivative that can be directly visualized. The label can also be detected, and the signal amplified, using our affinity-purified antibody conjugates to rhodamine. (Excitation maximum at 595 nm; emission maximum at 615 nm).
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