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Agarose bound, succinylated Wheat Germ Agglutinin (WGA)

Catalog Number: AL-1023S

Availability: In stock

Product Name Unit Size Price Qty
Agarose bound, succinylated Wheat Germ Agglutinin (WGA) 2 ml
$135.00
Agarose bound, succinylated Wheat Germ Agglutinin (WGA) 5 ml
$240.00

Agarose bound, succinylated Wheat Germ Agglutinin (WGA) Zoom

Overview

    Features:

    • Matrix is heat stable, cross-linked 4% agarose beads with a molecular exlusion of about 2x107 daltons
    • Bead diameter ranges in size from 45-165 microns
    • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
    • Immobilized lectins are prepared using affinity purified lectins
    • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
    • Hydrophilic spacer arm is inserted between the lectin and the matrix
    • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
    • No residual charges present after conjugation.  This minimizes non-specific binding to the matrix
    • Product supplied as a 1:1 suspension in buffer
    • 3 mg lectin/ml gel
    • Inhibiting/Eluting Sugar: Chitin Hydrolysate; or 500 mM N-acetylglucosamine with salt and/or acid elution generally required; or Glycoprotein Eluting Solution (ES-5100)

     

    Additional Information

    Catalog Number AL-1023S
    Unit Size 2 ml, 5 ml
    Country of Manufacture United States
    Applications Glycobiology, Affinity Chromatography
    Matrix Conjugate Lectins
    Sugar Specificity N-Acetylglucosamine
    Conjugate Agarose
Additional Info

    Details

    This derivative has been reported to have properties distinct from the native lectin. Evidence suggests that Succinylated Wheat Germ agglutinin does not bind to sialic acid residues, unlike the native form, but retains its specificity toward N-acetylglucosamine. Using conjugates of the native lectin and the succinylated form can provide a system to distinguish between sialylated glycoconjugates and those containing only N-acetylglucosamine structures.

    Agarose bound*, succinylated WGA is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.

    This coupling method provides several advantages over the traditional cyanogen bromide procedure:

    • Maximum carbohydrate binding activity of the coupled lectins is retained
    • Linkage is stable over a range of pH values
    • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
    • No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.

    Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

    Inhibiting/Eluting Sugar: Chitin Hydrolysate (Cat. No. SP-0090) or 500 mM N-acetylglucosamine with salt and/or acid elution generally required

    *3 mg lectin/ml gel

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